Welcome to the <em>Exxon Valdez</em> Oil Spill Trustee Council Home Oil Spill Facts Habitat Protection Restoration Projects Recovery Since 89
Restoration Projects

Marine Derived Nutrients Quarterly Tasks

2006 Quarterly Project Tasks:

1st Quarter Tasks (October 1, 2005 - December 31, 2005)
Status: Complete

Tasks to be performed:

October - December - Data analysis.

Progress report:

(Not Available)

2nd Quarter Tasks (January 1, 2006 - March 31, 2006)
Status: Complete

Tasks to be performed:

January - February - Workshop to dicuss reults of year two, and approach for year three. Review. Compare and contrast our results with other proposed efforts in Kenai River and Prince William Sound. March: Coordinate/communicate with other proposed MDN research in Kenai River and Prince William Sound. Prepare for sampling.

Progress report:

Food Web Sampling and Analysis: The objective for this portion of the project is to use stable isotopes, lipid, and fatty acid analysis as possible means for understanding how marine derived nutrients subsidize stream food webs in the continuum from headwaters to stream mouth. The isotope analyses indicates the presence and proportion of incorporated marine derived nutrients; the lipid and fatty acid analyses reveal the fitness effects of marine derived nutrients. Sample Preparation and Analysis: During this quarter, we refined the protocols for the analysis of sulfur isotopes, and began weighing out fish samples from 2004 for sulfur analysis. Fatty acid and lipid analysis was initiated during this quarter at the Auke Bay laboratory. It is anticipated that the fatty acid and lipid analysis for the 2005 fish and invertebrate samples will be completed early in the 3rd quarter of 2006. Ongoing analyses of fatty acids and C, N, and S stable isotopes will provide additional information on the utility of these tools for tracking MDN in watersheds and into the influence of watershed geology on MDN uptake and effects. 2006 Site Selection: We completed an Operation Plan, including the site selection for our 2006 field season, (Figure 1). The 2006 Operation plan, for year three of the project, focuses on enhancing our understanding fish as integrators of MDN, and further development of a nutrient proxy for salmon escapement. In addition to sampling Dolly Varden, we will also sample juvenile coho because they are more ubiquitous in salmon bearing streams than Dolly Varden, and therefore may be a more suitable monitoring tool. Our goal for year three is to sample streams with a range of salmon densities so that we can assess the relationship between MDN concentrations, which is related to the number of returning salmon, and juvenile fish response (lipid levels, ?3:?6 fatty acids, d15N, d13C ). The 2006 field work will be conducted in association with salmon enumeration projects on fifteen streams throughout south central Alaska (Figure 1, Table 1). The streams were selected as sample sites to obtain a range of salmon return densities, and ease of access. Salmon densities were estimated by dividing the salmon returns, (from enumeration projects) and dividing that number by stream length, calculated from USGS maps. Each stream will be sampled twice ? once before the salmon (chinook, sockeye and/or pink, but not coho) runs begin and once two-three weeks after peak spawning. We are not attempting to sample post-coho runs because they enter the systems too late in the year to sample an MDN response in juvenile fish. Both juvenile Dolly Varden and coho will be collected at each site.

3rd Quarter Tasks (April 1, 2006 - June 30, 2006)
Status: Complete

Tasks to be performed:

April - September: Sampling on treatment and control waterhseds. Final report preparation.

Progress report:

1. Work Performed- Food Web Sampling and Analysis. The objective for this portion of the project is to use stable isotopes, lipid, and fatty acid analysis as possible means for understanding how marine derived nutrients subsidize stream food webs in the continuum from headwaters to stream mouth. The isotope analyses indicates the presence and proportion of incorporated marine derived nutrients; the lipid and fatty acid analyses reveal the fitness effects of marine derived nutrients. Sample Preparation and Analysis: During this quarter, the fatty acid and lipid analysis for the 2005 fish and invertebrate samples were completed at Auke Bay laboratory, and the S stable isotope analysis for the 2004 fish samples was completed at the USGS Stable Isotope Laboratory. We are in the process of analyzing and interpreting those data sets. Once the fatty acid and lipid samples were completed by the Auke Bay laboratory, the remainder of the fish samples (homogenate) was sent to the Kachemak Bay Research Reserve, where we have been processing the samples for stable isotope samples. During this quarter, we completed processing the samples for C and N analysis, and plan to complete the processing for S analysis by the end of August. Analysis of these 2005 samples will provide additional information on the utility of these tools for tracking MDN in watersheds and into the influence of watershed geology on MDN uptake and effects. 2006 Sampling: During this quarter, we completed fish sampling on 13 salmon bearing streams with varying run densities. Our intention at the beginning of the field season was to sample 15 salmon bearing streams for this portion of the project. However, after repeatedly attempting sampling of Barabara Creek and Deshka River, we found no juvenile coho, and so these two streams were dropped from the sampling scheme. At each of the 13 streams, we collected and measured weight and length of all juvenile Dolly Varden and coho collected. Five Dolly Varden and five coho from each stream were kept for fatty acid and lipid and stable isotope analysis. During the next quarter, we will complete the post salmon run sampling for the 13 streams. 2. Work Performed - Water chemistry nutrient proxy. The objective for this portion of the project is to derive and fit models describing the relationship between the number of returning salmon and concentrations of ammonium (NH4+), nitrate (NO3-), soluble reactive phosphorous (SRP) in the water. 2006 Sampling: Weekly water chemistry sample collection began as weirs became operational on the Buskin, Deshka, Anchor and Russian Rivers. A video camera recording system was deployed on Humpy Creek on June 20 with the assistanve of the Alaska Department of Fish and Game, Commercial Fish Division, and weekly water samples and discharge measurements have been obtained since that time. We expect to continue water sample collection at all five sites until mid-September. During this quarter, we obtained high flow discharge on the Deshka River using the Reserve?s Acoustic Doppler Current Profiler (ADCP). The Statewide Aquatic Resources Coordination Unit of the Alaska Department of Fish and Game has been assisting with discharge measurements on the Deshka at low flows. We will use these discharge measurements to develop a rating curve for the Deshka. 3. Future Work. We anticipate beginning the final round of sampling for this project on September 15 and completing the sampling by September 30. 4. Coordination/Collaboration. This project was developed as a collaborative effort between researchers, agency and citizen monitoring groups, with the purpose of providing information towards the development of GEM?s long-term watershed monitoring program, and for the benefit of regional managers, and local coastal communities. We have continued actively working with five Alaska Department of Fish and Game salmon enumeration projects as part of the nutrient proxy development. We also continue to coordinate our work with Ron Heintz at the Auke Bay laboratory. 5. Community Involvement/TEK & Resource Management Applications/Information Transfer During this quarter, updates on this project were presented to the Reserve?s Community Council. 6. Budget There are no substantial differences between the expected and actual budget expenses.

4th Quarter Tasks (July 1, 2006 - September 30, 2006)
Status: Complete

Tasks to be performed:

April - September: Sampling on treatment and control waterhseds. Final report preparation.

Progress report:

See 2006 annual report.

Can't find it? We can help.

We're working to make all of our project information available online. Currently, we have 380 Final Reports and 811 Annual Reports available for download. If you would like more information on any project you find using this search engine, please contact us. We can search our files for the information you request.

Or, check with ARLIS.

The Alaska Resources Library and Information Service (ARLIS), located on the UAA campus, may have the document you need.

Phone: (907) 272-7547

reference@arlis.org

http://www.arlis.org/